Is Micrococcus luteus citrate positive or negative?

Is Micrococcus luteus citrate positive or negative?

Bacteria Collection: Micrococcus luteus Additional Information

Fermentation Tests Text: Maltose : -,Salicin : –
GramReaction Text: positive
Acid Fast Staining Text: negative
Motility At 25°C Text: negative
Emulsifiability Text: easy

What test determine Micrococcus luteus?


TEST Staphylococcus aureus Micrococcus luteus
Catalase Positive Positive
Glucose Fermentation Positive Negative
Coagulase Positive Negative
Salt Tolerance on Mannitol Salt Tolerance Positive Negative

Is Micrococcus luteus indole positive?

Micrococcus luteus. Micrococcus luteus was biochemically observed as positive to catalase, oxidase, methyl blue, Simmon’s citrate, gelatin liquefaction and aesculin, while ngative to indole, coagulase, methyl red, hydrogen peroxide and Voges Proskauer.

Is Micrococcus luteus gelatinase positive?

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Enzyme Tests Text: Beta-galactosidase : -,Catalase : +,Gelatinase : –
GramReaction Text: positive
Acid Fast Staining Text: negative
Motility At 25°C Text: negative
Emulsifiability Text: easy

How do you test for citrate?

Procedure of Citrate Utilization Test

  1. Streak the slant back and forth with a light inoculum picked from the center of a well-isolated colony.
  2. Incubate aerobically at 35 to 37 C for up to 4-7 days.
  3. Observe a color change from green to blue along the slant.

Does Micrococcus luteus have catalase positive?

Other distinguishing identification features are that M. luteus is urease & catalase positive but coagulase negative. Some Micrococcus species are now identified, particularly on newer identification techniques such as MALDI-TOF, as Kocuria sp.

What are the different tests used to differentiate micrococcus species from Staphylococcus species?

Micrococci may be distinguished from staphylococci by a modified oxidase test23,24. Their colonies are usually pigmented in shades of yellow or red and grow on simple media. The optimum growth temperature is 25-37°C.

Are all Micrococcus oxidase positive?

Characteristics. Micrococcus are aerobic, Gram-positive cocci ranging in size from 0.5 to 2.0 μm in diameter. They occur in pairs, tetrads or clusters but not in chains. They are catalase positive and often oxidase positive although this reaction may be weak (see Table 23.3).

Are all Micrococcus oxidase-positive?

Is Micrococcus luteus gamma hemolytic?

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Genus species Gram+ Micrococcus luteus
Morphology* G+ cocci/packets
Pigment* yes
Hemolysis* gamma

What indicates a positive gelatinase test?

Expected Results. Positive: Partial or total liquefaction of the inoculated tube (the control tube must be completely solidified) at 4°C within 14 days. On plates, gelatin hydrolysis is indicated by clear zones around gelatinase-positive colonies. Negative: Complete solidification of the tube at 4°C.

What is the gelatinase test?

The gelatin hydrolysis test detects the ability of bacteria to produce gelatinases. This test aids in the identification of Serratia, Pseudomonas, Flavobacterium and Clostridium.

What is Simmons citrate agar test?

Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen phosphate as the sole source of nitrogen, other nutrients, and the pH indicator bromthymol blue.

What is the purpose of Simmons citrate test?

Simmons Citrate agar is used to test an organism’s ability to utilize citrate as a source of energy. Ammonium Dihydrogen Phosphate is the sole source of nitrogen.

What rapid test is used to differentiate Streptococcus from Micrococcus?

Bacitracin Sensitivity This test is used for the rapid identification of group A streptococci. It can be used to distinguish staphylococci (resistant) from micrococci (sensitive).

Does M. luteus have cytochrome c oxidase?

luteus, and NADH oxidase and cytochrome c oxidase in cytoplasmic membranes of M. luteus. 100% activity of NADH oxidase was 0.0149mM NADH minute 1 and of cytochrome c oxidase 0.117 mM cytochrome c minute 1.

What type of hemolysis is Micrococcus?


Genus species Gram+ Morphology* Hemolysis*
Micrococcus luteus G+ cocci/packets gamma
Mycobacterium smegmatis G+ rod ND
Staphylococcus aureus G+ cocci/clusters beta
Staphylococcus epidermidis G + cocci gamma

Does Micrococcus luteus Grow on mannitol salt agar?

luteus causes odours in humans when breaking down the components of sweat. Mannitol Salt Agar can be used to culture Micrococcus spp as it is selective for certain Gram- positive microorganisms.

Is Staphylococcus epidermidis a gelatinase test?

The gelatinase test can be used to differentiate between Staphylococcus aureus and Staphylococcus epidermidis . It can also be used to differentiate Serratia marcescens, Proteus vulgaris, and Proteus mirabilis from other enterics.

What does a negative gelatin hydrolysis test mean?

Expected results. Gelatin Hydrolysis Test: Above tube: Positive. Below Tube: Negative. Positive: Partial or total liquefaction of the inoculated tube (uninoculated control medium must be completely solidified) even after exposure to the cold temperature of ice bath or refrigerator (4°C).

Which bacteria gives positive and negative result in Simmons citrate test?

List of Bacteria which gives positive and negative result in Simmons citrate test Microorganism Result Klebsiella pneumoniae Positive (+) Salmonella Typhi Negative (-) Enterobacter species Positive (+) Citrobacter freundii Positive (+)

What color is Micrococcus luteus?

Some of the species of Micrococcus, such as M. luteus which is yellow in colour, and M. roseus which is red. They also produce yellow or pink colonies when they are grown on the mannitol salt agar, micrococcus flavus, M. terreus, and M. yunnanensis.

Can Micrococcus luteus be isolated from blood during septic episodes?

Modern molecular typing of serially isolated micrococcal strains was not performed. We present a patient with rhabdomyosarcoma in whom a single clone of Micrococcus luteus was isolated from blood during septic episodes during a period of 7 weeks despite appropriate therapy.

How do you test for Micrococcus luteus?

1. Micrococcus Luteus Oxidase Test: The microdase test, also known as modified oxidase test is a rapid test to differentiate Staphylococcus from Micrococcus which are Gram-positive cocci possessing catalase enzymes. The differentiation is based on the detection of the oxidase enzymes.