Table of Contents
What is orbitrap used for?
The Orbitrap is frequently used in research applications to elucidate low-abundance, high complexity, or otherwise difficult samples, including those collected in proteomics, lipidomics, clinical research and forensic toxicology, food and beverage, and environmental laboratories.
What is orbitrap resolution?
Orbitrap analyzers exhibit high mass resolution (> 150,000) with 1–5 ppm mass accuracy [14]. However, this performance comes with a high price tag that is comparable to some TOF analyzers. Orbitrap analyzers utilize a central rod-like electrode surrounded by a barrel-shaped outer electrode.
What is orbitrap Fusion?
The Orbitrap Fusion Tribrid Mass Spectrometer enables scientists analyzing the most challenging low-abundance, high-complexity samples to identify more compounds faster, quantify more accurately and elucidate structures more thoroughly.
Why is orbitrap high resolution?
In a relatively small amount of time, compatible with ultra high performance liquid chromatography (UHPLC), very high-mass resolutions can routinely be achieved with the Orbitrap MS. High resolution allows us to discriminate between compounds that have very similar masses when measured in the mass spectrometer.
Why is Orbitrap high resolution?
High mass resolution is necessary to separate signals of one compound from those of another and ensure that ions of only one kind contribute to a particular measurement. The measurement may be an accurate-mass determination or a highly specific quantification measurement.
What are the three analyzers in a orbitrap fusion instrument?
The Orbitrap Fusion Lumos MS contains three mass analyzers, including quadrupole, Orbitrap, and LIT. Quadrupole mass analyzer (Q1) contains four circular rods.
What is tandem mass spectrometry used for?
Tandem mass spectrometry can be used for protein sequencing. When intact proteins are introduced to a mass analyzer, this is called “top-down proteomics” and when proteins are digested into smaller peptides and subsequently introduced into the mass spectrometer, this is called “bottom-up proteomics”.
What is the difference between mass spectrometry and tandem mass spectrometry?
A tandem mass spectrometry (TANDEM MS), also named as MS/MS, is a two-step technique used to analyze a sample either by using two or more mass spectrometers connected to each other or a single mass spectrometer by several analyzers arranged one after another.
What is Orbitrap mass analyzer?
In mass spectrometry, Orbitrap is an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle.
What is a spectrometer used for?
In the broadest sense a spectrometer is any instrument that is used to measure the variation of a physical characteristic over a given range; i.e. a spectrum.