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What are hk2 cells?
HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. The cell line appears to be derived from a single cell based on Southern and FISH analysis.
How many number of cell lines are available for purchase at ATCC?
ATCC has the world’s largest and most extensive product catalog of human and animal cell lines for research purposes. The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways.
Where is HEK293 from?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
What is the difference between 293 and 293T cells?
The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production. 293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .
What does HEK293 stand for?
human embryonic kidney cells
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
Why is HEK293 used for transfection?
Advantages of HEK293 cells Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.
What are the functional characteristics of hk-2 cells?
Furthermore, HK-2 cells retain functional characteristics of proximal tubular epithelium (Na+ dependent/phlorizin sensitive sugar transport; adenylate cyclase responsiveness to parathyroid, but not to antidiuretic, hormone). The E6/E7 genes are present in the HK-2 genome, as determined by PCR.
Can hk-2 cells be used as a tool to study injury and repair?
To assess its potential usefulness as a tool for studying injury and repair, HK-2 cells were exposed to a toxic concentration of H2O2 +/- iron chelation (deferoxamine) or hydroxyl radical scavenger (Na benzoate) therapy.
How do I import hk-2 cells to Hawaii?
Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required. Ryan MJ, et al. HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.
Is hk-2 a well-differentiated PTC line derived from adult human kidney?
However, no well-differentiated PTC line derived from adult human kidney currently exists. Therefore, … HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney Kidney Int. 1994 Jan;45(1):48-57.doi: 10.1038/ki.1994.6.